Plasmid_Backbone

Part:BBa_K3893016:Design

Designed by: Camila Gallegos   Group: iGEM21_Ecuador   (2021-10-21)


BioBricks compatible L4440 plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found at 2526
    Illegal suffix found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2526
    Illegal SpeI site found at 5
    Illegal PstI site found at 19
    Illegal NotI site found at 12
    Illegal NotI site found at 2532
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2526
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2526
    Illegal suffix found at 5
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2526
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2541
    Illegal SpeI site found at 5
    Illegal PstI site found at 19
    Illegal NgoMIV site found at 2245
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1069


Design Notes

It was changed with a PCR overhang that included the RFC10 prefix and suffix in order to convert it to our modular platform afterwards.


Source

We used AddGene plasmid L4440 as a base and added the prefix and suffix sequence with PCR overhang.

References